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Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

HSP70 protein plays an important role in protecting cells from deleterious stresses. Hsp70 is expressed constitutively in a wide variety of cells at normal growth temperatures. Its expression is induced by various physiological stresses, including exposure to heat shock and heavy metal. The activity of the HSP70 promoter can be induced by moderate hyperthermia (39°C to 43°C), reaching expression levels similar to those of the CMV promoter.

This premade lentivirus expresses GFP under the control of the HSP70 promoter

Virus: HSP70-GFP
Titer (approx): 1 x 108 CFU/ml
Vector Information: Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene: HSP70
Target Gene / Reporter(s): GFP
Selection Gene: Puromycin
Biosafety Level: BSL-2
Shipped: Dry ice
Storage: Store at -80°C

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