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PLV-10031-25

This set contains six EF1A-driven vectors: LIN28 (SKU PLV-10015), NANOG (SKU PLV-10014), SOX2 (SKUPLV-10013), OCT3/4 (SKU PLV-10012), KLF4 (SKU PLV-10011) and C-MYC (SKU PLV-10010). Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. EF-1 alpha (or EF1a) is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells).

Virus: set of lentivirus (EF1A-driven LIN28, NANOG, SOX2, OCT3/4, KLF4 and C-MYC)
Titer (approx): 1 x 108 CFU/ml
Vector Information: Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene: EF1A
Target Gene / Reporter(s):
Selection Gene: Puromycin
Biosafety Level: BSL-2
Shipped: Dry ice
Storage: Store at -80°C


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